In-vivo MR monitoring of magnetically labeled mesenchymal stem cells in kidneys after selective intrarenal injection in a glomerulonephritis model in rats at 3T
Ittrich H, Schaeffter T, Adam G, Leussler C, Nolte-Ernsting C, Thaiss F, Lange C, Dahnke H
University Hospital Hamburg Eppendorf
We evaluated MRI for in-vivo tracking of selective intraarterial injected superparamagnetic iron oxide particle-labeled mesenchymal stem cells (MSC) in a rat model of an Anti-Thy1 glomerulonephritis. 106 labeled rMSC were injected in one renal artery. In-vivo T2*weighted MR Imaging and T2* Relaxometry were performed repetitively up to day 22 in a clinical 3T scanner and correlated with histology. Significant SNR and T2* decrease was observed in kidney of injection side up to 3 weeks after administration in MSC group with glomerular predominance. Delayed MSC migration to liver and spleen was observed. The study demonstrates the potential of MRI for in-vivo cell-tracking of therapeutically administered, magnetically labeled MSC.