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Abstract #2140

Application of Functional Magnetic Resonance Imaging to the study of 5-HT2C Neurotransmission in Rat Brain

Davies K, Stark J, Williams S, Luckman S, McKie S
University of Manchester

We are investigating animal fMRI as a tool in discovering new appetite pathways to help develop anti-obesity agents. Here we investigated whether high field fMRI could detect receptor modulation by specific serotonin (5-HT1B and 5-HT2C) receptor antagonists, after stimulation by the anorexigenic mixed 5-HT receptor agonist m-chlorophenylpiperazine (mCPP) in the rat. Animals received either vehicle or mCPP, and a pre-treatment of receptor antagonist or its vehicle. Data were analysed in SPM2 by 1-way ANOVA followed by F tests. The results showed distinct cancellation of mCPP-induced activity by the 2C receptor antagonist. However the 1B antagonist had little effect.