Non-invasive Analysis of Gallbladder Bile Composition in Cynomolgus Monkeys using In Vivo 1H-MRS
Knnecke B, von Kienlin M
F. Hoffmann-La Roche Ltd
Bile is an essential compartment in lipid and cholesterol metabolism which serves the purpose of solubilising hydrophobic metabolites for excretion and dietary uptake. Biliary malfunction is implicated in numerous common diseases such as obesity. Hence, repetitive assessment of bile would be highly desirable. Here, we demonstrate in a cynomolgus monkey model that in vivo 1H-MRS provides high-resolution spectra which allow bile composition to be non-invasively and repetitively assessed in situ. Quantification reveals glycine and taurine conjugates of cholate, chenodeoxycholate and deoxycholate as the major biliary constituents. A cross-sectional and longitudinal study provides insight into the variability of bile composition.