Magnetic Resonance Spectroscopy: a Non-invasive Tool for Monitoring Histone Deacetylase Inhibition
Sankaranarayanapillai M, Tong W, Pal A, Bornmann W, Gelovani J, Ronen S
UT M.D.Anderson Cancer Center
Histone deacetylase (HDAC) inhibitors have emerged as a new class of anti-neoplastic agents having potential anti-tumor activities for targeted therapy. Our goal was to develop a non-invasive MRS based method to monitor HDAC inhibition in PC3 cells. We already showed that Boc-Lys-Tfa(OH) is an HDAC substrate. Here, 19F MRS of cells treated with p-fluoro-suberoylanilide hydroxamic acid (FSAHA) detected increased intracellular BLT levels which correlated with HDAC inhibition. 31P MRS of the FSAHA treated cells detected increased phosphocholine levels (PC) which also correlated with HDAC inhibition. This indicates that 19F and 31P MRS are promising modalities to assess HDAC inhibition.