Hyperpolarization by DNP is Applicable to 13C Isotopomer Analysis
Merritt M, Malloy C, Blazina D
UTSW Medical Center
A constant challenge facing magnetic resonance is the low sensitivity. The sensitivity is directly related to the relative populations of the spin energy levels probed by the experiment. Hyperpolarization by DNP at liquid helium temperatures followed by rapid dissolution has been shown to increase the polarization by orders of magnitude. To test the applicability of this method to 13C isotopomer analysis, we have carried out feasibility studies on uniformly 13C labeled glutamate and acetate. Initial experiments demonstrate that this method will work for the range of molecules of interest, and will not be confounded by multiple isotopomers.