Rapid and simultaneous analysis of all major constituents in human bile using 1H NMR spectroscopy
Ijare O, Nagana Gowda G, Somashekar B, Sharma A, Kapoor V, Khetrapal C
Centre of Biomedical Magnetic Resonance
One step analysis of major bile components in intact bile is difficult due to broad and overlapping NMR signals. This problem is overcome by using dimethylsulfoxide which results in resolved NMR signals. Using rigorous 1D and 2D NMR studies at 400 and 700 MHz, distinct markers for cholesterol, bile acids and glycerophospholipids were identified and used for quantitative analyses of major constituents of bile in a single step. Accurate, rapid and simultaneous analyses of major bile components shown here for the first time may have immense implications for pathophysiology, diagnosis and prognosis of biliary and intestinal diseases.