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Abstract #0080

Better Understanding Maturation Arrested Men Through HR-MAS Spectroscopy of Human Testicular Biospy Tissue

Rahwa Berhanu Iman1, Mark Swanson1, Thomas Walsh2, David Aaronson2, Shoujun Zhao, Ying Lu, John Kurhanewicz1

1Radiology, University of California, San Francisco, San Francisco, CA, USA; 2Urology, University of California, San Francisco

1H high resolution magic angle spinning (HR-MAS) spectroscopy was used to determine metabolic profiles of normal, maturation arrested, and azoospermatic human testicular biopsy tissues. Twenty seven 1H HR-MAS spectra were acquired and quantified using the electronic standard ERETIC and the algorithm HR-QUEST. Phosphocholine (PC), phosphoethanolamine (PE), and glutamine were found to significantly differentiate between normal and azoospermatic tissue. This study further suggests that PC could serve as a specific in vivo marker for fertility in 1H MRSI studies of human testes and a model can be used to predict fertility of maturation arrested men.