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Abstract #0520

Development of an in Vivo Functional Assay to Monitor the Effect of SPIO Labeling on Murine Dendritic Cells Used for Cell Therapy in MRI

Richard Tavar1, Gopal Varma1, Pervinder Sagoo2, Yakup Tanriver2, Tobias Schaeffter1, Robert Lechler2, Giovanna Lombardi2, Greg Mullen1

1Division of Imaging Sciences, King's College London, London, UK; 2Department of Nephrology and Transplantation, King's College London, London, UK

Dendritic cells are currently being studied as a therapy for both cancer and transplant tolerance. Non-invasive imaging of dendritic cells labeled with superparamagnetic iron oxides (SPIOs) migrating to lymph nodes allows for the monitoring of an efficient cellular therapy. Here, to test the funtion of labeled versus unlabeled cells, the development of an in vivo funtional assay is described, as well as serial imaging of SPIO labeled dendritic cells migrating to the popliteal lymph node.