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Abstract #0522

Tracking Enhanced Green Fluorescence Protein (EGFP) and Micrometer-Sized Particles of Iron Oxide (MPIO) Labeled Mesenchymal Stem Cells (MSCs) in a Myocardial Infarction Model with Granulocyte-Colony Stimulating Factor (GCSF) Modulation

Yidong Yang1,2, Yuhui Yang2, Brianna Klein2, Nathan Yanasak2, Xingming Shi3, William Dave Hill4,5, Tom C.-C. Hu1,2

1Medical Physics Program, Georgia Institute of Technology, Atlanta, GA, USA; 2Department of Radiology, Medical College of Georgia, Augusta, GA, USA; 3Department of Pathology, Medical College of Georgia, Augusta, GA, USA; 4Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, GA, USA; 5Veterans Affairs Medical Center, Medical College of Georgia, Augusta, GA, USA

Synopsis: Stem cells play an important role in tissue repair and regeneration. In this study, MSCs labeled with EGFP and MPIO were transplanted into the mouse bone marrow. It was observed using MRI that the labeled cells infiltrated and engrafted into the myocardial injury and adjacent site and lead to gradual signal attenuation. Furthermore, the GCSF potentially modulated the infiltration rate of labeled MSCs and enhanced the signal attenuation. Results from this study suggest a useful method for the development of drugs designed to modulate the stem cell mobilization process.