Resolution of H3proS and H3proR Deuterations in [2-13C]glutamate

Tiago Brandao Rodrigues¹, Ins Ribeiro Violante¹, Sebastin Cerdn¹

We provide a protocol to discriminate the ²H substitution of the H3_proR or H3_proS hydrogens of [2-¹³C]glutamate. Using ¹³C MRS at 18.8T, we detected the existence of two different isotopically shifted singlets in the glutamate C2 resonance of brain extracts of deuterated animals as derived from the deuterium substitutions in 3R or 3S, respectively. We show that the H3S depicted a lower value of vicinal isotopic shift (δ ₁=-0.058ppm) while the H3R originated the higher-field resonance (δ ₁=-0.071ppm), measured relatively to the [2-¹³C]glutamate C2 resonance.