Ping Wang1, Jason Moore1, Thomas Floyd1,2, Max Kelz1
1Anesthesiology & Critical Care, University of Pennsylvania, Philadelphia, PA, USA; 2Neurology, University of Pennsylvania, Philadelphia, PA, USA
Manganese is a useful contrast agent for MRI of animals, resulting in enhancement on T1-weighted MRI images. Two emerging hypothalamic targets with proven ability to affect arousal state are the median preoptic nucleus, MnPO, and ventrolateral preoptic nucleus, VLPO. Depolarization of these two regions is respectively thought to underlie onset and maintenance of natural sleep. Our hypothesis is that volatile anesthetics cause unconsciousness (behavioral hypnosis) by affecting VLPO and MnPO function. In this preliminary study, we verified the hypothesis by high-resolution MEMRI (manganese enhanced MRI) technique.