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Abstract #0875

Early Detection of Sepsis by Quantifying SPIO Uptake by LPS-Activated Macrophages Using COSMOS

Richard Wong1,2, Tian Liu1,2, Jian Shou3, Philip S. Barie3, Yi Wang1,2

1Biomedical Engineering, Cornell University, Ithaca, NY, USA; 2Department of Radiology, Cornell University - Weill Medical College, New York, NY, USA; 3Department of Surgery, New York-Presbyterian Hospital - Weill Cornell Medical Center, New York, NY, USA

This study offers an in vitro proof of concept; RAW264.7 murine monocytes were treated with lipopolysaccharide to induce a sepsis-like cell condition, were incubated with the FDA-approved contrast agent Feridex IV, and were imaged using the COSMOS technique (Calculation of Susceptibility through Multiple Orientation Sampling) for the quantification of iron. Results indicate that statistically significant differences can be found in SPIO uptake between activiated and control cells, indicating a potential use for COSMOS in the in vivo diagnosis of sepsis.