Abstract #1281

Effect of Dietary Composition on the Neuronal Activation Following Peripheral Injection of Cholecystokinin in Mice Detected by Manganese-Enhanced Magnetic Resonance Imaging

Nadine Zeeni^1,2, Nachiket A. Nadkarni³, Jimmy D. Bell³, Ralph Sinkus⁴, Gilles Fromentin^1,2, Daniel Tome^1,2, Nicolas Darcel^1,2

¹CNRH-IdF, UMR914 Nutrition Physiology and Ingestive Behavior, AgroParisTech, Paris, France; ²CNRH-IdF, UMR914 Nutrition Physiology and Ingestive Behavior, INRA, Paris, France; ³Metabolic and Molecular Imaging Group, Imaging Sciences Department, MRC Clinical Sciences Centre, Hammersmith Hospital, Imperial College London, London, UK; ⁴Laboratoire des Ondes et Acoustique, ESPCI, Universite Paris VII, Paris, France

We looked at the effect of long-term nutrient intake on the central response to CCK by manganese-enhanced MRI (MEMRI). C57BL/6 mice were fed a standard high-carbohydrate (HC), high-fat (HF) or high-protein (HP) diet for 6 weeks. Assessment of brain response to CCK showed a reduction in neuronal activity in appetite centers (VMN) and in reward centers (nucleus accumbens and striatum). When comparing diet effects, while the HF diet didnt induce any change in activity, reductions in Mn uptake were found in the PVN when comparing the HP to the HC diet. Our results suggest that habituation to a HP diet leads to modifications in CCK-induced anorectic effects in the hypothalamus.