Abstract #1287

Segmentation and Calculation of Stroke- And Manganese-Enhanced Volumes After Reconstruction of the Injured Hemisphere in the Hypoxic-Ischemic Neonatal Rat Brain

ystein Olsen¹, Marius Widere², Pl Erik Goa^3,4, Jon Skranes^2,5, Ann-Mari Brubakk^2,5, Christian Brekken³

¹Department of Radiography, Sr-Trndelag University College, Trondheim, Norway; ²Department of Laboratory Medicine, Childrens and Womens Health, University of Science and Technology, Trondheim, Norway; ³Department of Circulation and Medical Imaging, Norwegian University of Science and Technology,, Trondheim, Norway; ⁴Department of Radiology, St Olavs Hospital, Trondheim, Norway; ⁵Department of Pediatrics, St Olavs Hospital, Trondheim, Norway

The injured brain hemisphere was reconstructed based on the segmented non-injured intracranial volume and non-injured brain hemisphere. Animals with no Hypoxic-Ischemic injury (HI) were used for validation and a non-significant mean difference of 1.3% between the segmented and reconstructed hemisphere volumes was detected. This indicated a good correspondence between the direct segmented and reconstructed hemisphere. The semi-automatic method developed for this task enables calculation of absolute and relative stroke- and ME volumes and can be used for in vivo longitudinal study of HI development and associated manganese enhancement.