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Abstract #2291

Interrogation of Fluctuating Tumour Microvasculature Using Combined Carbogen-USPIO (CUSPIO) Imaging

Jake Samuel Burrell1, John C. Waterton2, Anderson Ryan2, Simon P. Robinson1, Simon Walker-Samuel1

1The Institute of Cancer Research, London, Sutton, UK; 2AstraZeneca, Cheshire, UK

Combined carbogen-USPIO (CUSPIO) imaging was used to investigate microvascular fluctuations SW1222 and HCT116 colorectal xenografts. CUSPIO exploits the inherent size difference in the mechanism of action of carbogen (95% O2, 5% CO2) and USPIO imaging (erythrocytes compared to nano-particles). Small vessels allow favourable entry to the USPIO particles. By producing δR2* maps temporal changes in vascular perfusion can be evaluated. This technique revealed a difference in the vascular stability of the two tumour types which has important implications for drug trials. This result suggests that CUSPIO imaging is a potentially useful method for imaging transient vascular shutdown in vivo.