Orlando Lopez1, Damian J. Tyler2, Kieran Clarke2, Edward Lakatta3, Richard G. Spencer1
1Magnetic Resonance Imaging and Spectroscopy Section, National Institute on Aging/NIH, Baltimore, MD, USA; 2University of Oxford, Department of Physiology Anatomy and Genetics, Oxford, UK; 3Laboratory of Cardiovascular Science, National Institute on Aging/NIH, Baltimore, MD, USA
Use of short repetition time (TR) relative to metabolite T1s is common in 31P MRS of biological samples. However, spectral resonances acquired with short TR values exhibit saturation effects that have traditionally been corrected using saturation factors. This approach does not account for the chemical exchange exhibited in biological systems which may lead to large errors. Here, we describe an approach to metabolite quantification based on performing ongoing dualangle measurements (O-DAM) in the setting of chemical-kinetic changes. Results showed that O-DAM correction can permit accurate monitoring of metabolite concentrations even in the setting of chemical exchange and changing chemical-kinetic parameters.
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