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Abstract #2430

Imaging Carbonic Anhydrase Activity Using Hyperpolarized 13C-Labeled Bicarbonate

Ferdia Aidan Gallagher1,2, Mikko I. Kettunen2,3, Rebekah L. McLaughlin2,3, De-en Hu2,3, Tim H. Witney2,3, Jan H. Ardenkjaer-Larsen4, Kevin M. Brindle2,3

1Radiology, University of Cambridge, Cambridge, UK; 2CRUK Cambridge Research Institute, Cambridge, UK; 3Biochemistry, University of Cambridge, Cambridge, UK; 4GE Healthcare, Amersham, UK

Carbonic anhydrase (CA) is of biological importance because tumor-associated CAs (e.g. CA IX) have been shown to be strongly induced by hypoxia and controlled by hypoxia-inducible factor-1 (HIF-1). Therefore imaging CA may directly relate to hypoxia and HIF-1 activation. We show here that CA activity can he imaged both in vitro and in vivo following the injection of hyperpolarized 13C-labeled bicarbonate by saturating the 13C-labeled carbon dioxide. This represents a new application of Dynamic Nuclear Polarization.