Pratip Bhattacharya1, Shawn Wagner1, Henry R. Chan1, Eduard Y. Chekmenev1, William H. Perman2, Brian D. Ross3
1Enhanced MR Laboratory, Huntington Medical Research Institutes, Pasadena, CA, USA; 2School of Medicine, St. Louis University, St. Louis, MO, USA; 3Enhanced MR Laboratory, Huntington Medical Research Institutes, Pasadena, Pasadena, CA, USA
Several significant biological and biomedical questions have been addressed by use of 15N MR. However, structural 15N NMR is hampered by the need for complex chemical substitutions and 15N enriched biomedical applications which are time consuming, limited by enzyme flux measurements of mmoles over seconds to minutes. Hyperpolarization of 15N by Parahydrogen And Synthesis Allows Dramatically Enhanced Nuclear Alignment (PASADENA) and Dynamic Nuclear Polarization (DNP) was explored as a means of broadening the biomedical utility of 15N MR imaging and spectroscopy. This abstract describes the hyperpolarization of several 15N molecules with both PASADENA and DNP techniques and their potential in vivo applications.