Hideo Sato-Akaba1, Hirotada Fujii2, Hiroshi Hirata1
1Division of Bioengineering and Bioinformatics, Hokkaido University, Sapporo, Hokkaido, Japan; 2School of Health Sciences, Sapporo Medical University, Sapporo, Hokkaido, Japan
To visualize the distribution of free radicals with a short lifetime, the temporal resolution of three-dimensional (3D) EPR imaging should be several times shorter than the lifetime of the free radicals. The purpose of the present work was to further reduce the acquisition time for 3D EPR imaging to improve the temporal resolution. An acquisition time of 5.8 s was achieved under conditions of 46 projections and a field-scan frequency of 9 Hz. This is 5 times faster than that previously reported for a 650-MHz CW-EPR imaging apparatus, which makes it possible to acquire 3D projection data in 30 s.
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