Michael David Wong1, Sharon Portnoy1, Christine Lalibert1, R Mark Henkelman1
1Mouse Imaging Centre, Hospital for Sick Children, Toronto, Ontario, Canada
Active staining of fixed mouse brain with gadolinium based contrast agents can drastically improve image quality. Our study demonstrates a new active staining protocol where the concentration of contrast agent during perfusion is proportional to the ratio of brain volume to brain blood volume. With this protocol the brains reach equilibrium with the contrast agent approximately 2 times faster than with traditional active staining protocols.
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