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Abstract #3137

19F MRI of Fibroblasts and Neuroblastoma Cells Labeled with Emulsified Perfluoro-15-Crown-5 Ether

Samir Mulla-Osman1, Ute Bommerich2, Johannes Bernarding3

1IBMI, University of Magdeburg, Magdeburg, Sachsen-Anhalt, Germany; 2Leibniz-Institute for Neurobiology, Germany; 3IBMI, University of Magdeburg, Sachsen-Anhalt, Germany


19F-labelled cells provide an important new technique to evaluate in vivo the migration of implanted cells such as in stem cell therapy. 19F-marker substances such as perfluorocarbons (PFC ) have been used for cell tracking based on 19F spectroscopy as well as 19F magnetic resonance imaging (MRI). We present first results of 19F MRI of fibroblasts and neuroblastoma cells labeled with PFCE emulsion. The results of the present study show that the labelling procedure allows a hight transfection of the cells. The concentration of the PFCE emulsion reached high levels and cells can be successfully monitored using 19F-MR Imaging.

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