Contrast Quantification of MPIO Labeled Cell Migration in the OB of Mice as a Read Out for the Efficiency of in Situ Labeling Strategies

Ruth Vreys¹, Marleen Verhoye^1,2, Annemie Van der Linden¹

We describe a method for contrast quantification based on threshold segmentation of hypointense voxels. The method uses four VOIs to be able to correct for signal bias between the OB ipsilateral and OB contralateral to the injection and to correct for false positive hypointense voxels originating from other sources than MPIOs. The results revealed contrast accumulation for two out of four strategies to label eNPC in mice by intraventricular MPIO injections. This method could be useful for quantification of neurogenesis capacity.