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Abstract #3162

Endosomal Escape of Gd-Agents Activated by External Photochemical Stimulus

Eliana Gianolio1, Francesca Arena1, Anders Hogset2, Silvio Aime1

1Chemistry IFM, University of Torino, Torino, Italy; 2PCI Biotech AS, Oslo, Norway

The novel Photochemical internalisation (PCI) technology, that promotes the release of endocytosed molecules into cells cytosol, has been succesfully adapted to MRI applications in order to improve the efficiency of Gd-containing probes internalized by cells through pinocytotic mechanism. The release of endocytosed paramagnetic probes into the cytosol removes the relaxivity quenching produced by the entrapment of high concentrations of Gd-complexes into endosomes thus traducing in a great enhancement in signal intensity if compared with the case of endosomes compartimentalized agents.