Song I. Chun1, Tae Hyung Kim1, Kee Chin Tan1, Min Young Choi2, Jee Hyun Cho3, Kwan Soo Hong3, Jung Woog Shin1, Ok Chan Jung1, Young Il Yang4, Chi Woong Mun1
1Dept. of Biomedical Engineering, Inje University, Gimhae, Korea; 2Paik Institute of Clinical Reserch, Inje University, Busan, Korea; 3Korea Basic Science Institute, Ochang, Korea; 4Dept.of Pathology, Paik Hospital, Inje University, Busan, Korea
The purpose of this study is to observe cell metabolism with MRS when a MDSCs is differentiated into fat. Three experimental groups used MDSCs that cultured in 3 dimensional system, Group1(fibrin gel), Group2(fibrin+undifferentiated MDSCs: cultured 1day, 1week), Group3(fibrin+ differentiated MDSCs: cultured 1week). The spectrum from each group has been acquired by utilizing vertical-bore 14.1T NMR/MRI with PRESS pulse sequence. Compare to spectrums of group 1, 2 and 3, we analyzed metabolite peaks newly formed during the differentiation of the MDSCs. In the result, the common peaks at 3.7/3.5/1.8/1.22/0.8ppm have been detected at each spectrum. Group 3, cultured MDSCs for 1 week into fat, came out a new peak at 2.6ppm and the increase of lipid peaks were also shown. In this study, therefore, we could observe the metabolite change along with MDSCs differentiation and found the potential possibilities of MRS to evaluate the differentiations of stem cell.
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