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Abstract #4043

Metabolic Characterization of Primary Human Colorectal Cancers Using High Resolution Magic Angle Spinning 1H Magnetic Resonance Spectroscopy

Martial Piotto1,2, Franois-Marie Moussallieh3,4, Baudouin Dillmann4, Alessio Imperiale, Agnes Neuville, Cecile Brigand, Jean-Pierre Bellocq, Karim Elbayed, Izzie Namer

1Bruker Biospin, Wissembourg, France; 2Strasbourg University, Strasbourg, Strasbourg, France; 3University Hospitals of Strasbourg, Strasbourg, France; 4Strasbourg University, Strasbourg, France

Colorectal cancer is one of the most frequent and most lethal forms of cancer in the western world. The aim of this study is to characterize by 1H high resolution magic angle spinning NMR spectroscopy (HRMAS) the metabolic fingerprint of both tumoral and healthy tissue samples obtained from a cohort of patients affected by primary colorectal adenocarcinoma. By analyzing HRMAS data using multivariate statistical analysis (PLS-DA), the two types of tissues could be discriminated with a high level of confidence. The identification of the metabolites at the origin of this discrimination revealed that adenocarcinomas are richer in taurine, glutamate, aspartate and lactate whereas healthy tissues contain a higher amount of myo-inositol and β-glucose.