Meeting Banner
Abstract #4372

Investigating DNP Markers for Stem Cell Tracking at Low Field

Li Sze Chow1, Behrouz Aflatoonian2, Harry D. Moore2, Martyn N.J. Paley1

1Academic Unit of Radiology, University of Sheffield, Sheffield, UK; 2Centre for Stem Cell Biology, Department of Biomedical Sciences, University of Sheffield, Sheffield, UK

This study investigated the use of a low field (8.2mT, 348kHz NMR frequency) dedicated MR system for Dynamic Nuclear Polarization (DNP) with 239MHz ESR frequency. The experiments have demonstrated the feasibility of imaging plant transpiration of 3-Carbomyl-PROXYL. We have also investigated stem cells tracking marked with a DNP tracer. Thus far no enhancement has been observed from the stem cell clusters but it could be due to the low number of stem cell clusters and thus large partial volume effect. There is also a possibility that the C-Proxyl may not have crossed the cell membranes or it had reacted with the culture medium.