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Abstract #4496

Using the Linewidth of the Amide Proton CEST Effect of MRI-PARACEST Agents for PH Mapping

Alex Xuexin Li1,2, Craig K. Jones1, Mojmir Suchy1,3, Robert H.E. Hudson3, Ravi S. Menon1,2, Robert Bartha1,2

1Center for Functional and Metabolic Mapping, Robarts Research Institute, London, ON, Canada; 2Department of Medical Biophysics, The University of Western Ontario, London, ON, Canada; 3Department of Chemistry, The University of Western Ontario, London, ON, Canada

A technology to measure pH using the amide protons site of a thulium complex with a DOTAM-Glycine-Lysine ligand: Tm3+-DOTAM-Gly-Lys was developed. The pH can be determined uniquely from the linewidth of the asymmetry curve of the CEST spectrum in a manner independent of contrast agent concentration, intrinsic magnetization transfer effect, and temperature for a given saturation pulse. pH maps with standard deviation less than 0.1 pH units were obtained from 10 mM Tm3+-DOTAM-Gly-Lys solutions with different pH.