Evaluation of the Relationship Between LSO2 MR Measurement and Hypoxia : Impact of an Antiangiogenic Treatment on a Gliosarcoma Model

Benjamin Lemasson¹, Thomas Christen^1,2, Raphal Serduc³, Cecile Maisin¹, Audrey Bouchet³, Christopoh Segebarth¹, Graldine Le Duc³, Chantal Rmy¹, Emmanuel Louis Barbier¹

Despite a highly vascular phenotype, most glioblastomas cells are in hypoxia. Monitoring of hypoxia could be useful for monitoring the effectiveness of anti-tumor therapies. In this study, we evaluate (i) the relationship between the oxygenation (lSO₂) estimated by MRI and tissue hypoxia estimated by immunohistology and (ii) the impact of an antiangiogenic (Sorafenib) treatment on the vasculature (Blood volume fraction; BVf) and the lSO₂ of gliosarcoma model (9L). lSO₂ estimate by MRI was correlated to tumor hypoxia observed by immunohistochimistry. Results of this study also suggest that lSO₂ could be a sensitive reporter of the hypoxic effects of antiangiogenic therapies.