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Abstract #0209

Comparison of Rate of Islet Loss in Syngeneic, Allogeneic and Xenogeneic Grafts in Rat Using Quantification of Iron Oxide Labeled Islet Cells by 3D Radial UTE MRI.

Lindsey Alexandra Crowe1, Frederic Ris2, Sonia Nielles-Vallespin3, Peter Speier3, Michel Kocher4, Solange Masson2, Christian Toso2, Domenico Bosco2, Thierry Berney2, Jean-Paul Valle1

1Department of Radiology, Geneva University Hospital, University of Geneva, Faculty of Medicine, Geneva, Switzerland; 2Cell Isolation and Transplant Center, Department of Surgery, Geneva University Hospital, Geneva, Switzerland; 3Siemens AG Medical Solutions, Erlangen, Germany; 4Biomedical Imaging Group, School of Engineering, Ecole Polytechnique Fdrale de Lausanne, Lausanne, Vaud, Switzerland

In-vivo 3D difference ultra-short echo time (dUTE) imaging gives quantitative positive contrast images for serial examination by automatic segmentation of iron oxide labeled islet cell clusters transplanted into the liver. Coverage of the whole liver in the absence of cardiac and respiratory motion artifact, and isotropic resolution is obtained with uniform background suppression. Three types of grafts: syngeneic, allogeneic and xenogeneic, were studied over time in rat, with success of islet graft, effect of magnetofection and rate of islet loss measurably different. The method shows promise for robust long term tracking of cell rejection in patients.