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Abstract #0416

Comparison of in Vivo Post-Exercise PCr Recovery and Basal ATP Synthesis Flux for the Assessment of Skeletal Muscle Mitochondrial Function

Nicole Martina Adriana van den Broek1, Jolita Ciapaite1, Klaas Nicolay1, Jeanine J. Prompers1

1Biomedical NMR, Department of Biomedical Engineering , Eindhoven University of Technology, Eindhoven, Netherlands

The interpretation of basal ATP synthesis flux (VATP) measured by 31P saturation transfer (ST) is not straightforward. In this study, post-exercise PCr recovery and ST-based VATP were compared in a rat model of mitochondrial dysfunction. Treatment with complex 1 inhibitor DPI induced mitochondrial dysfunction, as evidenced by a decreased oxygen consumption rate in isolated mitochondria and a decreased in vivo post-exercise PCr recovery. Interestingly, no significant difference in VATP was observed between DPI-treated rats and controls. This shows that ST measurements in rest do not necessarily reflect intrinsic mitochondrial function, but more likely the ATP demand of the cell.