Simon Hu1, Asha Balakrishnan2,
Robert Bok1, Peder E. Larson1, Sarah J. Nelson1,
John Kurhanewicz1, Andrei Goga2, Daniel B. Vigneron1
1Dept. of Radiology and Biomedical
Imaging, University of California, San Francisco, San Francisco, CA, United
States; 2Dept. of Medicine, Division of Hematology/Oncology,
University of California, San Francisco, San Francisco, CA, United States
Development
of hyperpolarized technology utilizing dynamic nuclear polarization has
enabled the monitoring of 13C metabolites in vivo at very high SNR. In this work, hyperpolarized 13C
3D-MRSI was used to measure liver metabolism in mice after expression of the
MYC proto-oncogene was switched on and then off in the liver. Mice in various
disease stages were studied, and significant differences in hyperpolarized
lactate and alanine levels were detected (P < 0.01). In addition,
biochemical assays showed increased LDH expression and activity in the
MYC-driven tumors.
Keywords