Meeting Banner
Abstract #0657

Hyperpolarized [1-13C] Pyruvate Metabolism in a Human Prostate Tissue Culture Bioreactor

David J. Joun1, Mark Albers1, Kayvan Keshari1, Robert Bok1, Christopher Ward, Donna Peehl2, Sabrina Ronin, Daniel Vigneron, John Kurhanewicz

1Radiology, UCSF, San Francisco, CA, United States; 2Urology, Stanford, Stanford, CA, United States

We demonstrate for the first time that the pathologic and metabolic integrity of benign and malignant human prostate tissues can be maintained in a NMR compatible 3-D tissue culture bioreactor for 32 hours. After administration of hyperpolarized [1-13C] pyruvate, the generation of labeled hyperpolarized lactate and LDH activity was significantly higher in malignant tissues (N=3) relative to benign human prostate tissues (N=3). Moreover, there was minimal overlap of the labeled hyperpolarized lactate signal between individual cancer and benign tissues suggesting that hyperpolarized lactate will be an accurate biomarker of prostate cancer in patients.