In Vivo Hepatic Localized Proton Magnetic Resonance Spectroscopy at 7T in a Glycogen Storage Disease Mouse Model

Nirilanto Ramamonjisoa¹, Hlne Ratiney¹, Fabienne Rajas², Elodie Mutel², Frank Pilleul^1,3, Olivier Beuf¹, Sophie Cavassila¹

In vivo 1H magnetic resonance spectroscopy (MRS) was used to evaluate the hepatic steatosis in a mouse model of GSD1a under two different diets, a standard- and a high fat diet. Accumulation of hepatic fat and fat composition within the liver were assessed. The estimated MRS profiles for both groups (Figure 2) showed significant differences for the lipid methyl resonances at 0.9ppm. Both estimated levels of the methylene resonances (1.3ppm) were significantly higher than the estimates obtained for control mice fed on standard diet. Based on MR imaging observations, 90% of the mice fed on high-fat diet exhibited adenomas in the liver while none fed on standard diet. These measurements will give insight into the understanding of the onset and progression of adenomas in a mouse model of GSD1a under different diets