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Abstract #0872

Immune Responses to Adeno Associated Virus Vectors in Canine Muscle Using MRI for Duchenne Muscular Dystrophy

Donghoon Lee1, Martin Kushmerick1, Zejing Wang2, Stephen Tapscott2

1University of Washington, Seattle, WA, United States; 2Fred Hutchinson Cancer Research Center, Seattle, WA, United States

One challenge of gene therapy, a promising treatment for Duchenne muscular dystrophy, is to understand immune responses to adeno associated virus (AAV) vectors used for gene delivery. Canine MR imaging was conducted to noninvasively monitor local inflammatory responses to AAV in dog muscle over time by measuring T2 values and volumes of the inflammatory areas. The volume increase of 33 ~ 150 % was monitored on semitendinosus muscle and the median T2 value was significantly higher at the sites of AAV injection (60.1 5.4 ms) than those from the un-injected muscles (33.6 0.5 ms) in the contra-lateral muscle.