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Abstract #0926

Acute Effect of Gamma Irradiation in Mice by NMR Based Metabolic Profiling of Urine

Ahmad Raza Khan1, Poonam Rana1, M Memita Devi1, Shubhra Chaturvedi2, Subash Khushu1

1NMR Research Centre, INMAS, Delhi, India; 2Division and Cyclotron & Radiopharmaceutical Sciences, INMAS, Delhi, India


A high resolution 1H NMR spectroscopy based metabonomic approach has been used to study acute effect of gamma irradiation at biochemical levels. Urine samples were collected from mice at 6, 24 and 96 hrs post irradiation with dose of 3, 5 and 8 Gy. Significant changes were observed in high dose of gamma irradiation even after 6 hrs, while maximum changes observed in low and moderate dose after 24 hrs of exposure. These alterations in metabolites could be helpful for identification of potential biomarkers associated with radiation induced changes and may find applications in biological dosimeters.

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