feng zhang1, Huidong Gu1, Yanfeng Meng1, Bensheng Qiu1, Xiaoming Yang1
1Image-Guided Bio-Molecular Intervention Section, Department of Radiology, University of Washington School of Medicine, Seattle, WA, United States
MR imaging was used to investigate the capability of Motexafin gadolinium(MGd) entering human cholangiocarcinoma cells (Mz-ChA-1) and the feasibility of intrabiliary local delivery of MGd into the common bile duct(CBD) wall. T1 weighted MR imaging of Mz-ChA-1 cells treated with MGd demonstrated a linear increase of signal intensities(SI) from 25 to 75-g/mL MGd, and a plateau pattern of SIs from 75 to 150-g/mL MGd. Confocal microscopy showed MGd internalized Mz-ChA-1 cells as intracytoplasm pink dots. Ex vivo experiments revealed significant higher contrast-to-noise ratio in the MGd-infused CBD walls than that in the controlled CBD walls with phosphate-buffered saline.