Abstract #2768

Metabolic Profile of Lipid Extracts Obtained by Astrocytic Brain Tumors

Frauke Nehen¹, Wieland Willker¹, Laura Columbano², Rudolf Fahlbusch², Dieter Leibfritz¹

¹Institute of Organic Chemistry, University of Bremen, Bremen, Germany; ²International Neuroscience Institute Hannover, Hannover, Germany

Lipophilic tissue extracts of astrocytic brain tumors were analyzed by ¹H-NMR spectroscopy. Tumor core, tumor margin and reference tissue differ significantly with respect to their content of galactosyl cerebrosides and one unknown metabolite. Lipid separation with solid phase extraction of eight lipid extracts, enrichment by combining fractions in which the unknown metabolite was observed and analysis by various 2D-NMR methods revealed that the unknown metabolite is an isoprene derivative.