Anant Bahadur Patel1, Puneet Bagga1, K.S. Varadarajan1, T.V. Rohith1
1NMR Microimaging and Spectroscopy,
Centre for Cellular and Molecular Biology,
In this study we have used a novel approach of co-infusion of [U-13C6]glucose and [2-13C]acetate in mice to study neuronal and astroglial metabolism simultaneously. 1H-[13C]- and 13C-[1H]-NMR spectroscopy were used to analyze the neurotransmitter turnover from labeled substrates in different brain regions. Half time for GluC4 and GABAC2 increased in the order; Cortex-SubCortex-Cerebellum while that of GlnC4 from [2-13C]acetate is in the order; SubCortex-Cortex-Cerebellum. Further, the initial synthesis rate of glutamate and GABA from glucose decreased in the order: Cortex-SubCortex-Cerebellum while that of glutamine from acetate increased in the order: Cortex-SubCortex-Cerebellum.