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Abstract #3357

Simultaneous Measurement of Neuronal and Astroglial Metabolism in Mouse Brain

Anant Bahadur Patel1, Puneet Bagga1, K.S. Varadarajan1, T.V. Rohith1

1NMR Microimaging and Spectroscopy, Centre for Cellular and Molecular Biology, Hyderabad, Andhra Pradesh, India

In this study we have used a novel approach of co-infusion of [U-13C6]glucose and [2-13C]acetate in mice to study neuronal and astroglial metabolism simultaneously. 1H-[13C]- and 13C-[1H]-NMR spectroscopy were used to analyze the neurotransmitter turnover from labeled substrates in different brain regions. Half time for GluC4 and GABAC2 increased in the order; Cortex-SubCortex-Cerebellum while that of GlnC4 from [2-13C]acetate is in the order; SubCortex-Cortex-Cerebellum. Further, the initial synthesis rate of glutamate and GABA from glucose decreased in the order: Cortex-SubCortex-Cerebellum while that of glutamine from acetate increased in the order: Cortex-SubCortex-Cerebellum.