R. Matthew Hutchison1,2, Seyed M. Mirsattari3, Craig K. Jones4, Joseph S. Gati1, L. Stan Leung5
1Robarts Research Institute, University of Western Ontario, London, Ontario, Canada; 2Graduate Program in Neuroscience, University of Western Ontario, London, Ontario, Canada; 3Clinical Neurological Sciences, University of Western Ontario; 4F.M. Kirby Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States; 5Physiology and Pharmacology, University of Western Ontario
This work is an examination of the spatio-temporal dynamics of low-frequency hemodynamic fluctuations of the anesthetized rat brain. Applying independent component analysis to baseline BOLD fMRI timecourses, synchronous bilateral cortical and subcortical networks were observed including: primary and secondary somatosensory cortices; motor cortices; striate cortices; posterior and anterior cingulate; hippocampi; caudate putamen; and thalamic nuclei. Networks were preserved under two different anesthetic regimes: isoflurane and ketamine/xylazine. This technique has allowed a complete exploration of the resting networks in the rat brain that was not afforded by previously used correlational techniques that have been used for rat imaging.