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Abstract #3755

The Use of Cellular MRI and Magnetic Particles to Study Cancer Stem Cells

Emeline Julie Ribot1, Carmen Simedrea2, Patricia McGowan2, Ann Chambers2, Paula J. Foster1

1Imaging Laboratories, Robarts Research Institute, London, Ontario, Canada; 2Medical Biophysics, University of Western Ontario, London, Ontario, Canada

In this abstract, we describe technology developed in our labs for tracking stem-like cancer cells (CSC), in a mouse model of breast cancer metastasis to the brain, using MRI and magnetic particles. A human breast cancer cell line was sorted by flow cytometry into two distinct populations: CD44high/CD24low and CD44low/CD24high, representing the CSC-like and non-CSC cells, respectively. The sorted cell populations can be labeled efficiently and without toxicity with the iron agent MoldayION Rhodamine B. Labeled CSC can be detected in vivo in images of the mouse brain after injection into the left ventricle of the heart in nude mice.