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Abstract #4618

Alterations on Hepatic Glycogen and Lipid Metabolism Following the Induction of Diabetes in the Rat

Ana Francisca Soares1,2, John Griffith Jones1, Francisco Veiga2, Rui Albuquerque Carvalho1

1Life Sciences, Faculty of Sciences and Technology and Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal; 2Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal

The liver stores carbohydrate and lipid after a meal under insulin stimulation. Using deuterated water, we assessed hepatic glycogen synthesis and de novo lipogenesis in healthy and streptozotocin-induced diabetic rats feeding overnight. Hepatic glycogen and lipid content were analyzed by 2H-NMR. Healthy animals showed similar direct and indirect pathway contributions to glycogen. Following Diabetes induction we observed a progressive loss of direct pathway capacity compared to the indirect and also a reduction of de novo lipogenesis. These observations may serve as valuable markers for assessing alterations in hepatic glucose and lipid metabolism during the progress Diabetes.