Ana Francisca Soares1,2, John Griffith Jones1, Francisco Veiga2, Rui Albuquerque Carvalho1
1Life Sciences, Faculty of Sciences and Technology and Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal; 2Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal
The liver stores carbohydrate and lipid after a meal under insulin stimulation. Using deuterated water, we assessed hepatic glycogen synthesis and de novo lipogenesis in healthy and streptozotocin-induced diabetic rats feeding overnight. Hepatic glycogen and lipid content were analyzed by 2H-NMR. Healthy animals showed similar direct and indirect pathway contributions to glycogen. Following Diabetes induction we observed a progressive loss of direct pathway capacity compared to the indirect and also a reduction of de novo lipogenesis. These observations may serve as valuable markers for assessing alterations in hepatic glucose and lipid metabolism during the progress Diabetes.