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Abstract #0019

Quantitative Assessment of Mitochondrial Metabolic Efficiency by 17O & 31P MR Spectroscopy in Isolated Rat Hearts

Bharath Atthe1, 2, Mary Kemerer1, 2, Ya Chen1, 2, Ming Lu1, 2, Gheorghe Mateescu2, 3, Chris Flask2, 3, Xin Yu1, 2

1Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA; 2Case Center for Imaging Research, Case Western Reserve University, Cleveland, OH, USA; 3Radiology, Case Western Reserve University School of Medicine, Cleveland, OH, USA

In this study, we investigated the potential of combining 17O spectroscopy (17O-MRS) and 31P magnetization transfer (31P-MT) method for evaluating metabolic efficiency in perfused hearts. ATP synthesis rate was determined from 31P-MT experiments, while mitochondrial oxidation was quantified by the rate of H217O production. 2,4-dinitrophenol (DNP) was used to induce the uncoupling of mitochondrial oxidation and phosphorylation. Our results suggest that the ratio of ATP synthesis measured by 31P-MT to mitochondrial H217O production quantified by 17O-MRS can be used as an indicator of metabolic efficiency.