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Abstract #0035

A New Method for Phenotyping the Brain Tumor Microenvironment using MR Microscopy

Eugene Kim1, Jiangyang Zhang2, Karen Hong3, Arvind P. Pathak2,4

1Department of Biomedical Engineering, the Johns Hopkins University School of Medicine, Baltimore, MD, USA; 2Russell H. Morgan Department of Radiology & Radiological Science, the Johns Hopkins University School of Medicine; 3The Johns Hopkins University School of Public Health; 4JHU ICMIC Program

The development of pre-clinical brain tumor models and anti-angiogenic therapies has created a critical need to characterize the brain tumor microenvironment. Here we describe a novel method for whole-brain 3D mapping of the vasculature of a mouse brain tumor model using magnetic resonance microscopy (μMRI). The vascular architecture was characterized by six morphological parameters. Region-of-interest analysis showed significant differences in these parameters between tumor and contralateral brain. In combination with diffusion-weighted MRI, we could characterize the phenotypes of post-inoculation day-12 and day-17 tumors. These results demonstrate the feasibility of using μMRI to characterize microenvironmental changes that accompany brain tumor progression.