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Abstract #0240

Mn Distribution in Rat Hippocampus: Correlative use of Synchrotron X-Ray Microprobe & MEMRI

Alexia Daoust1,2, Emmanuel Luc Barbier1,2, Sylvain Bohic1,3

1INSERM U836, Grenoble, France; 2Grenoble Institut des Neurosciences, Universit Joseph Fourier, Grenoble, France; 3European Synchrotron Radiation Facility (ESRF), Grenoble, France

In MEMRI protocols, the cellular distribution remains still unclear. To obtain further insights, we imaged rat brains by MRI after IP and IC MnCl2 injections. Then, using synchrotron X-ray microprobe, we mapped the distribution of Mn, Ca, Zn and Fe in rat hippocampus. The hippocampal distributions of Mn obtained by both techniques were in excellent agreement. At a cellular scale, Mn was distributed within the DG/hilus/CA3. After IC injection, Mn was preferentially located in hippocampal fissure, a structure rich in astrocytes. The presence of Mn also modifies the distribution of Fe and Zn.