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Abstract #0313

Hepatic Glycogen Metabolism in Mice by In Vivo 13C MRS at 14T

Christine Nabuurs1, Frederic Preitner2, Bernard Thorens2, Rolf Gruetter3

1CIBM, Hpitaux Universitaires de Genve (HUG), Lausanne, Switzerland; 2Mouse Metabolic Facility, Center for Integrative Genomics, UNIL, Lausanne, Switzerland; 3Laboratory of Functional & Metabolic Imaging (LIFMET), l'Ecole Polytechnique Fdrale de Lausanne (EPFL), Lausanne, Switzerland

Contributions of direct and indirect glycogen synthesis in liver tissue can be determined by 13C MRS upon labeled glucose infusion. We optimized a protocol for in vivo MRS at 14T in mouse liver to study the incorporation of 13C into different positions of glycogen by overcoming the large bandwidth needed for simultaneous detection of signals with a chemical shift difference of 6kHz by applying interleaved transmitter frequencies. This allowed for sufficient time resolution and omits signal losses and the need for pulse shape corrections when studying alternative pathways of glycogen synthesis in mouse models with disorders in hepatic carbohydrate metabolism.