Albert P. Chen1, Ralph E. Hurd2, Marie A. Schroeder3,4, Angus Z. Lau4,5, Yi-Ping Gu4, Wilfred W. Lam4, Jennifer Barry4, James Tropp6, Charles H. Cunningham4,5
1GE Healthcare, Toronto, ON, Canada; 2GE Healthcare, Menlo Park, CA, USA; 3Department of Physiology, Anatomy & Genetics, University of Oxford, Oxford, United Kingdom; 4Imaging Research, Sunnybrook Health Sciences Centre, Toronto, ON, Canada; 5Deptartment of Medical Biophysics, University of Toronto, Toronto, ON, Canada; 6GE Healthcare, Fremont, CA, USA
Utilization of either C1 or C2 labeled pre-polarized pyruvate as a tracer can only afford a partial view of cardiac pyruvate metabolism. If pyruvate was labeled at both C1 and C2 positions, then it would be possible to observe the down stream metabolites that were the results of both PDH and Krebs cycle flux with a single tracer bolus. Intracellular pH could also be estimated from the same data, provided the 13CO2 signal has adequate SNR. This study demonstrated the feasibility of simultaneous investigation of cardiac PDH flux, Krebs cycle metabolism and intracellular pH in vivo, by using hyperpolarized [1,2-13C2]pyruvate.