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Abstract #0863

Hepatic Fatty Acid Quantification using MRS & GC in a Mouse Model of GSD1A Under Two Different Diets

Nirilanto Ramamonjisoa1, Helene Ratiney1, Elodie Mutel2, Herve Guillou3, Gilles Mithieux2, Frank Pilleul1,4, Fabienne Rajas2, Olivier Beuf1, Sophie Cavassila1

1CREATIS, CNRS UMR 5220, Inserm U1044, INSA-Lyon, Universit Lyon 1, Universit de Lyon, Lyon, France; 2Inserm U855, Universit Lyon 1, Universit de Lyon, France; 3INRA ToxAlim Integrative Toxicology & Metabolism, Toulouse, France; 4Imagerie Digestive - CHU, Hospices Civils de Lyon, France

Glycogen storage disease type I (GSD1) is an autosomal recessive metabolic disorder resulting in severe impairment of glucose production and large accumulation of liver fatty acids. In this paper, short-TE in vivo MRS and gas chromatography (GC) analysis were used to evaluate the fatty liver content and the fat fraction composition in the mouse model of GSD1a under two different diets, a chow and a high fat high sucrose diet. Strong agreements between the quantitative lipid profiles measured by MRS and GC were demonstrated. Our results confirm that 1H MRS is a suitable non invasive tool for hepatic lipid quantification and lipid fraction estimation.