Nirilanto Ramamonjisoa1, Helene Ratiney1, Elodie Mutel2, Herve Guillou3, Gilles Mithieux2, Frank Pilleul1,4, Fabienne Rajas2, Olivier Beuf1, Sophie Cavassila1
1CREATIS, CNRS UMR 5220, Inserm U1044, INSA-Lyon, Universit Lyon 1, Universit de Lyon, Lyon, France; 2Inserm U855, Universit Lyon 1, Universit de Lyon, France; 3INRA ToxAlim Integrative Toxicology & Metabolism, Toulouse, France; 4Imagerie Digestive - CHU, Hospices Civils de Lyon, France
Glycogen storage disease type I (GSD1) is an autosomal recessive metabolic disorder resulting in severe impairment of glucose production and large accumulation of liver fatty acids. In this paper, short-TE in vivo MRS and gas chromatography (GC) analysis were used to evaluate the fatty liver content and the fat fraction composition in the mouse model of GSD1a under two different diets, a chow and a high fat high sucrose diet. Strong agreements between the quantitative lipid profiles measured by MRS and GC were demonstrated. Our results confirm that 1H MRS is a suitable non invasive tool for hepatic lipid quantification and lipid fraction estimation.