1H-MRS Detects Differences of Carnosine Profile in Skeletal Muscle of Rats Fed with High-Fat & Placebo Diets

Yew S. K. Terry¹, Arunima Pola¹, Bhaskaran David Prakash¹, Mehdy Ghaeminia¹, S. S. Velan¹

Carnosine is a dipeptide found in high concentrations in the skeletal muscle. In this study, F344 rats on high fat diet [n=4] and placebo diet [n=2] were scanned over a period of 12 weeks using a Bruker 7T ClinScan MRI/MRS scanner. Over a period of 17 weeks, the supplementation of high fat diet containing histidine (0.5%) apart from other amino acids caused a slight upregulation of carnosine content in the rats compared to their control, fed CE2. Histidine supplementation upregulates carnosine content in the rats EDL skeletal muscle and provides additional information on diet induced changes to metabolism.