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Abstract #1395

In Vivo Absolute Quantification for Mouse Muscle Metabolites using an Inductively Coupled Synthetic Signal Injection Method & Newly Developed 1H/31P Dual Tuned Probe

Donghoon Lee1, Kenneth Marro1, Mark Mathis1, Eric Shankland1, Cecil Hayes1

1University of Washington, Seattle, WA, United States

We obtained robust estimates of 31P metabolite content in mouse muscles using a synthetic signal injection method and an optimized, 1H/31P dual tuned probe. 31P metabolite concentrations obtained from normal mice were well within the expected range reported in the literature. Our 1H/31P dual tuned probe was composed of two separate solenoid coils and each solenoid included a second coil to allow injection of pseudo signals. In addition to in vivo 31P metabolite quantitation, 1H MR imaging was conducted for both legs to perform parametric MRI along with muscle volume measurements.