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Abstract #1583

TRPV1-Mediated Entry of QX-314 Leads to Inhibition of Nociceptive Input as Measured by BOLD fMRI in Mice using Thermal Stimulation

Simone Claudia Bosshard1, Florian Stuker1, Constantin von Deuster1, Markus Rudin1,2

1Institute for Biomedical Engineering, University & ETH Zurich, Zurich, Switzerland; 2Institute of Pharmacology & Toxicology, University Zrich, Zurich, Switzerland

To study nociceptive processing in mice, a thermal stimulation paradigm using an infrared laser source for forepaw stimulation was developed. The skin of the forepaws was heated to 45C or 46C for 60s, using a feedback system to control the temperature. The stimulation led to significant BOLD changes in the somatosensory cortex and the thalamus. For modulation, a combination of QX-314 and capsaicin was injected into the forepaws, inducing a selective nociceptor block, which led to an abolishment of the BOLD signal. Either solution applied alone did not lead to a decrease in BOLD signal changes.